Chitin nanocrystals prepared by oxidation of α-chitin using the O2/laccase/TEMPO system.
Carbohydr Polym
; 189: 178-183, 2018 Jun 01.
Article
em En
| MEDLINE
| ID: mdl-29580396
ABSTRACT
Laccase mediator oxidation was applied to chitin at pH 6.8 and 30⯰C to prepare chitin nanocrystals with a catalytic amount of 2,2,6,6-tetramethylpiperidine-1-oxyl radical (TEMPO). When 40â¯mM TEMPO and a total of 500â¯U laccase were added to 1â¯g chitin, the yield of water-insoluble oxidized chitin was more than 95%, and the carboxylate content was 0.43â¯mmol/g. Adsorption of laccase molecules on chitin particles occurred in a buffer at pH 6.8, which may have been caused by electrostatic interactions between positively charged C2-ammonium groups of chitin and anionically charged groups of laccase. Rod-like chitin nanocrystals (ChNCs) were obtained with average lengths and widths of 480⯱â¯200â¯nm and 24⯱â¯17â¯nm, respectively, by sonication of the oxidized chitin/water suspensions. The O2/laccase/TEMPO oxidation caused no decrease in the degree of N-acetylation or the crystallinity of the original chitin based on FTIR and X-ray diffraction data.
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MEDLINE
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En
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2018
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Article