Thioredoxin-Dependent Decomposition of Protein S-Nitrosothiols.
Methods Mol Biol
; 1747: 281-297, 2018.
Article
em En
| MEDLINE
| ID: mdl-29600467
ABSTRACT
The addition of nitric oxide to cysteine moieties of proteins results in the formation of S-nitrosothiols (SNO) that have emerged as important posttranslational signaling cues in a wide variety of eukaryotic processes. While formation of protein-SNO is largely nonenzymatic, the conserved family of Thioredoxin (TRX) enzymes are capable of selectively reducing protein-SNO. Consequently, TRX enzymes are thought to provide reversibility and specificity to protein-SNO signaling networks. Here, we describe an in vitro methodology based on enzymatic oxidoreductase and biotin-switch techniques, allowing for the detection of protein-SNO targets of TRX enzymes. We show that this methodology identifies both global and specific protein-SNO targets of TRX in plant cell extracts.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Tiorredoxinas
/
Processamento de Proteína Pós-Traducional
/
S-Nitrosotióis
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article