ATRA attenuate proteinuria via downregulation of TRPC6 in glomerulosclerosis rats induced by adriamycin.
Ren Fail
; 40(1): 266-272, 2018 Nov.
Article
em En
| MEDLINE
| ID: mdl-29619864
ABSTRACT
OBJECTIVE:
In this research, we explored the molecular mechanism of proteinuria in glomerulosclerosis rats and the protective effects of ATRA.METHODS:
This research set up three groups SHO group, GS group, and ATRA group (15 mg/(kg d), Sigma, St. Louis, MO). The serum creatinine (Scr), urea nitrogen (BUN), and 24-h proteinuria were detected 12 weeks after administration of ATRA. The pathological and ultrastructure changes were observed under light microscope and transmission electron microscope. The protein expression of TGF-ß1 and Col-IV in glomerulus was detected by immunohitochemistry method. The mRNA and the protein expression of glomerular TRPC6 were detected by RT-PCR and Western blot.RESULTS:
In the rat model of GS, the expressions of TRPC6 were significantly elevated compared with the normal rat group; however, the use of ATRA down-regulated the expression of TRPC6 in the glomeruli and attenuated glomerulosclerosis and proteinuria. Scr and BUN were also improved by the treatment of ATRA.CONCLUSIONS:
Our results demonstrated that ATRA could ameliorate glomerulosclerosis and proteinuria in GS, which may be related to suppressed expression of TRPC6.Palavras-chave
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Base de dados:
MEDLINE
Assunto principal:
Proteinúria
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Tretinoína
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Glomerulosclerose Segmentar e Focal
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Canais de Cátion TRPC
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article