Importance of a diphosphorylated intermediate for RppH-dependent RNA degradation.
RNA Biol
; 15(6): 703-706, 2018.
Article
em En
| MEDLINE
| ID: mdl-29619898
ABSTRACT
Deprotection of the 5' end appears to be a universal mechanism for triggering the degradation of mRNA in bacteria and eukaryotes. In Escherichia coli, for example, converting the 5' triphosphate of primary transcripts to a monophosphate accelerates cleavage at internal sites by the endonuclease RNase E. Previous studies have shown that the RNA pyrophosphohydrolase RppH catalyzes this transformation in vitro and generates monophosphorylated decay intermediates in vivo. Recently, we reported that purified E. coli RppH unexpectedly reacts faster with diphosphorylated than with triphosphorylated substrates. By using a novel assay, it was also determined that diphosphorylated mRNA decay intermediates are abundant in wild-type E. coli and that their fractional level increases to almost 100% for representative mRNAs in mutant cells lacking RppH. These findings indicate that the conversion of triphosphorylated to monophosphorylated RNA in E. coli is a stepwise process involving sequential phosphate removal and the transient formation of a diphosphorylated intermediate. The latter RNA phosphorylation state, which was previously unknown in bacteria, now appears to define the preferred biological substrates of E. coli RppH. The enzyme responsible for generating it remains to be identified.
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MEDLINE
Assunto principal:
RNA Bacteriano
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Hidrolases Anidrido Ácido
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Estabilidade de RNA
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Proteínas de Escherichia coli
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Escherichia coli
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article