Injectable self-crosslinking HA-SH/Col I blend hydrogels for in vitro construction of engineered cartilage.
Carbohydr Polym
; 190: 57-66, 2018 Jun 15.
Article
em En
| MEDLINE
| ID: mdl-29628260
ABSTRACT
The injectable self-crosslinking blend hydrogel by combination of collagen I and thiolated hyaluronic acid could alleviate collagen I contraction in vitro and overcome weak cell adhesive sites of hyaluronic acid. Five groups of injectable hydrogels with different ratios were prepared to investigate their gelation time, injection force, mechanical properties, swelling capacity and disintegration performance. These results indicated that Col7HA-SH3 hydrogel achieved the optimal controlled and injectable effect, the gelation time was just ten seconds with injection force at 3.5â¯N, and the storage modulus of hydrogel could reach 11â¯kPa with frequency at 10â¯Hz. Furthermore, the phenotype maintaining, biocompatibility and chondrocytes proliferation were administrated by CLSM, SEM, histological staining, immuohistochemical staining, MTT test and glycoaminoglycans quantification. Similarly, the Col7HA-SH3 blend hydrogel encapsulated chondrocytes presented most excellent proliferation potential, phenotype maintaining, biocompatibility and convenient operational characteristics. These findings might approach the underlying clinical application of blend hydrogel in cartilage repair.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Materiais Biocompatíveis
/
Cartilagem
/
Hidrogéis
/
Colágeno Tipo I
/
Engenharia Tecidual
/
Ácido Hialurônico
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article