Evaluation of HPV-16 and HPV-18 specific antibody measurements in saliva collected in oral rinses and merocel® sponges.
Vaccine
; 36(19): 2705-2711, 2018 05 03.
Article
em En
| MEDLINE
| ID: mdl-29631883
ABSTRACT
BACKGROUND:
Current Human papillomavirus (HPV) L1 VLP vaccines protect against HPV-16 and HPV-18-associated cancers, in females and males. Although correlates of protection have not been identified, HPV-specific antibodies at sites of infection are thought to be the main mechanism of protection afforded by vaccination. Oral sampling has gained increased attention as a potential alternative to serum in monitoring immunity to vaccination and understanding local immunity in oral cancers.METHODS:
Serum was collected via venipuncture, and saliva was collected via oral rinses and Merocel® sponges from healthy volunteers 16 unvaccinated females, 6 females (ages 24-41) and 6 mid-adult aged males (ages 27-45) recipients of three doses of the HPV-16/18/6/11 vaccine (Gardasil®). Mid-adult male vaccine trial participants were compared to female participants. Samples were tested for anti-HPV-16 and anti-HPV-18 immunoglobulin G levels by an L1 virus-like particle-based enzyme-linked immunosorbent assay (ELISA).RESULTS:
All vaccinated participants had detectable serum anti-HPV-16 and anti-HPV-18 antibodies. Optimal standard concentration range and sample serial dilutions for oral rinses were determined. The standard curve was not affected by the type of solution examined. Reproducibility of HPV-16 and HPV-18 antibody titers in mouthwash (overall CVâ¯<â¯10%) or in Merocel® extraction buffer was robust (CVâ¯<â¯13%). Excellent assay linearity (R2â¯>â¯0.9) was observed for sera spiked controls in both solutions. HPV-16 and HPV-18 specific antibodies were detectable in saliva from vaccine recipients, both in mouthwash and in Merocel® sponges but levels were several logs lower than those in serum.CONCLUSIONS:
This study confirms the application of HPV-16 and HPV-18 ELISAs currently used in sero-epidemiological studies of immunogenicity of HPV vaccines for use with oral samples. Oral samples may be a useful resource for the detection of HPV-16 and HPV-18-specific antibodies in saliva following vaccination.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Saliva
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Manejo de Espécimes
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Papillomavirus Humano 16
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Papillomavirus Humano 18
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Anticorpos Antivirais
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article