Effective intracellular delivery and Th1 immune response induced by ovalbumin loaded in pH-responsive polyphosphazene polymersomes.
Nanomedicine
; 14(5): 1609-1618, 2018 07.
Article
em En
| MEDLINE
| ID: mdl-29649590
ABSTRACT
A polymersome system for delivering protein antigen ovalbumin (OVA) based on amphiphilic polyphosphazene grafting with N,N-diisopropylethylenediamine (DPA) and poly(ethylene glycol) (PEG) groups (poly[(DPA)m (PEG)n phosphazene], PEDP) was designed and constructed. The 200-240â¯nm-size OVA-loaded polymersomes displayed high stability at physiological pH, slow internalization through clathrin-mediated endocytosis pathway, and then a pH-triggered sustained OVA release in acidic environment, leading to extensive antigen access to cytosol. Prime-boost vaccine kept high antibody titers for 8â¯weeks and the subcutaneous vaccine of OVA polymersomes biased the immune response towards a type 1â¯T helper (Th1) response. Animal experiment results showed that the antigen-specific prophylactic vaccination by PEDP polymersomes delivery was much more rapid and efficient in depressing tumor growth and progress when compared with the therapeutic vaccination. These results suggested that PEDP-based polymersomes are very promising in controlled cytosolic delivery of protein antigens, and enhanced Th1 specific immune response.
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Base de dados:
MEDLINE
Assunto principal:
Compostos Organofosforados
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Polímeros
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Portadores de Fármacos
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Ovalbumina
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Sistemas de Liberação de Medicamentos
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Células Th1
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Linfoma
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article