Inactivation of ceramide synthase 2 catalytic activity in mice affects transcription of genes involved in lipid metabolism and cell division.
Biochim Biophys Acta Mol Cell Biol Lipids
; 1863(7): 734-749, 2018 07.
Article
em En
| MEDLINE
| ID: mdl-29653252
ABSTRACT
The replacement of two consecutive histidine residues by alanine residues in the catalytic center of ceramide synthase 2 in a new transgenic mouse mutant (CerS2 H/A) leads to inactivation of catalytic activity and reduces protein level to 60% of the WT level. We show here by qRT-PCR and transcriptome analyses that several transcripts of genes involved in lipid metabolism and cell division are differentially regulated in livers of CerS2 H/A mice. Thus, very long chain ceramides produced by CerS2 are required for transcriptional regulation of target genes. The hepatocellular carcinomata previously described in old CerS2 KO mice were already present in 8-week-old CerS2 H/A animals and thus are caused by the loss of CerS2 catalytic activity already during early life.
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Base de dados:
MEDLINE
Assunto principal:
Divisão Celular
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Carcinoma Hepatocelular
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Esfingosina N-Aciltransferase
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Metabolismo dos Lipídeos
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Neoplasias Hepáticas
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article