Development of a recombinant σB protein based dot-ELISA for the diagnosis of avian reovirus (ARV).
J Virol Methods
; 257: 69-72, 2018 07.
Article
em En
| MEDLINE
| ID: mdl-29660384
ABSTRACT
Avian reovirus (ARV) causes significant economic losses to the poultry industry worldwide. The ARV proteins fall into three different classes based on their sizesλ (large); µ (medium) and σ (small). σB, an outer capsid protein of the ARV contains group specific neutralizing epitopes and induces strong immune response in naturally infected chickens. This study describes the development of a rapid dot-enzyme linked immunosorbent assay (dot-ELISA) using recombinant σB protein antigen of 54â¯kDa (approx). The assay is rapid (4-5â¯h) and results can be read by the naked eye. Sixteen ARV positive serum samples (group A) produced strong reaction in the dot-ELISA while twenty of the ARV negative serum samples (group B) collected from SPF chickens showed no reaction. Seventy six randomly collected serum samples were tested with a commercial indirect ELISA kit and the in-house developed dot-ELISA. A total of sixty eight serum samples were found to be positive by indirect ELISA and sixty five serum samples were found to be positive by dot-ELISA. Therefore, using the commercial ELISA as the reference test, the dot-ELISA had a diagnostic sensitivity of 83.8% and specificity of 88.6%. This dot-ELISA can be used as a simple, reliable and inexpensive alternative to commercial ELISA kits for serodiagnosis of ARV where the facilities for standard ELISA are not available.
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Base de dados:
MEDLINE
Assunto principal:
Doenças das Aves Domésticas
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Ensaio de Imunoadsorção Enzimática
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Infecções por Reoviridae
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Orthoreovirus Aviário
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Anticorpos Antivirais
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Antígenos Virais
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article