Simultaneous analysis of multiple urinary biomarkers for the evaluation of oxidative stress by automated online in-tube solid-phase microextraction coupled with negative/positive ion-switching mode liquid chromatography-tandem mass spectrometry.
J Sep Sci
; 41(13): 2743-2749, 2018 Jul.
Article
em En
| MEDLINE
| ID: mdl-29679453
ABSTRACT
This study described an automated online method for the simultaneous determination of 8-isoprostane, 8-hydroxy-2'-deoxyguanosine, and 3-nitro-l-tyrosine in human urine. The method involves in-tube solid-phase microextraction using a Carboxen 1006 PLOT capillary column as an extraction device, followed by liquid chromatography with tandem mass spectrometry using a CX column and detection in the negative/positive switching ion-mode by multiple reaction monitoring. Using their stable isotope-labeled internal standards, each of these oxidative stress biomarkers showed good linearity from 0.02 to 2.0 ng/mL. Their detection limits (S/N = 3) were 3.4-21.5 pg/mL, and their intra- and inter-day precisions (relative standard deviations) were >3.9 and 6.5% (n = 5), respectively. This method was applied successfully to the analysis of urine samples, without any other pretreatment and interference peaks.
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Base de dados:
MEDLINE
Assunto principal:
Tirosina
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Dinoprosta
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Cromatografia Líquida
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Estresse Oxidativo
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Desoxiguanosina
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Espectrometria de Massas em Tandem
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Microextração em Fase Sólida
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article