Setup, Validation, and Quality Control of a Centralized Whole-Genome-Sequencing Laboratory: Lessons Learned.
J Clin Microbiol
; 56(8)2018 08.
Article
em En
| MEDLINE
| ID: mdl-29695528
ABSTRACT
Routine use of whole-genome analysis for infectious diseases can be used to enlighten various scenarios pertaining to public health, including identification of microbial pathogens, relating individual cases to an outbreak of infectious disease, establishing an association between an outbreak of food poisoning and a specific food vehicle, inferring drug susceptibility, source tracing of contaminants, and study of variations in the genome that affect pathogenicity/virulence. We describe the setup, validation, and ongoing verification of a centralized whole-genome-sequencing (WGS) laboratory to carry out sequencing for these public health functions for the National Infection Services, Public Health England, in the United Kingdom. The performance characteristics and quality control metrics measured during validation and verification of the entire end-to-end process (accuracy, precision, reproducibility, and repeatability) are described and include information regarding the automated pass and release of data to service users without intervention.
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Base de dados:
MEDLINE
Assunto principal:
Doenças Transmissíveis
/
Sequenciamento Completo do Genoma
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Laboratórios
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article