Abundance of enterovirus C in RD-L20B cell culture-negative stool samples from acute flaccid paralysis cases in Nigeria is geographically defined.
J Med Microbiol
; 67(6): 854-865, 2018 Jun.
Article
em En
| MEDLINE
| ID: mdl-29708482
ABSTRACT
PURPOSE:
We recently showed that enteroviruses (EVs) andenterovirus species C (EV-C) in particular were abundant in faecal samples from children who had been diagnosed with acute flaccid paralysis (AFP) in Nigeria but declared to be EV-free by the RD-L20B cell culture-based algorithm. In this study, we investigated whether this observed preponderance of EVs (and EV-Cs) in such samples varies by geographical region.METHODOLOGY:
One hundred and eight samples (i.e. 54 paired stool suspensions from 54 AFP cases) that had previously been confirmed to be negative for EVs by the WHO-recommended RD-L20B cell culture-based algorithm were analysed. The 108 samples were made into 54 pools (27 each from North-West and South-South Nigeria). All were subjected to RNA extraction, cDNA synthesis and the WHO-recommended semi-nested PCR assay and its modifications. All of the amplicons were sequenced, and the enteroviruses identified, using the enterovirus genotyping tool and phylogenetic analysis.RESULTS:
EVs were detected in 16 (29.63â%) of the 54 samples that were screened and successfully identified in 14 (25.93â%). Of these, 10 were from North-West and 4 were from South-South Nigeria. One (7.14â%), 2 (14.29â%) and 11 (78.57â%) of the strains detected were EV-A, EV-B and EV-C, respectively. The 10 strains from North-West Nigeria included 7 EV types, namely CV-A10, E29, CV-A13, CV-A17, CV-A19, CV-A24 and EV-C99. The four EV types recovered from South-South Nigeria were E31, CV-A1, EV-C99 and EV-C116.CONCLUSION:
The results of this study showed that the presence of EVs and consequently EV-Cs in AFP samples declared to be EV-free by the RD-L20B cell culture-based algorithm varies by geographical region in Nigeria.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Paraplegia
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Enterovirus Humano C
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Infecções por Enterovirus
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Fezes
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article