Tyrosinase inhibitory components from the seeds of Cassia tora.
Arch Pharm Res
; 41(5): 490-496, 2018 May.
Article
em En
| MEDLINE
| ID: mdl-29721815
ABSTRACT
Ten compounds (1-10) isolated from the seeds of Cassia tora were evaluated for tyrosinase inhibition. Compounds 3, 4, and 7 inhibited tyrosinase enzymatic activity in a dose-dependent manner, with IC50 values of 3.0 ± 0.8, 7.0 ± 0.4, and 9.2 ± 3.4 µM, respectively. Kinetic analyses revealed a mechanism consistent with competitive inhibition. In silico molecular docking showed that compounds 3 and 4 docked in the active site of tyrosinase, whereas 7 interacted with Ala246 and Val248 at outside of the active site, and His244 and Glu256 at inside. Additionally, compounds 3, 4, and 7 suppressed melanogenesis in α-MSH-treated B16F10 melanoma cells at a concentration of 10 µM.
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Base de dados:
MEDLINE
Assunto principal:
Sementes
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Extratos Vegetais
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Monofenol Mono-Oxigenase
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Cinnamomum aromaticum
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Inibidores Enzimáticos
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article