Selective determination of the catalytic cysteine pK<sub>a</sub> of two-cysteine succinic semialdehyde dehydrogenase from Acinetobacter baumannii using burst kinetics and enzyme adduct formation.

Phonbuppha, Jittima; Maenpuen, Somchart; Munkajohnpong, Pobthum; Chaiyen, Pimchai; Tinikul, Ruchanok

Selective determination of the catalytic cysteine pK_a of two-cysteine succinic semialdehyde dehydrogenase from Acinetobacter baumannii using burst kinetics and enzyme adduct formation.

Phonbuppha, Jittima; Maenpuen, Somchart; Munkajohnpong, Pobthum; Chaiyen, Pimchai; Tinikul, Ruchanok.

Afiliação

Phonbuppha J; Department of Biomolecular Science and Engineering, School of Biomolecular Science and Engineering, Vidyasirimedhi Institute of Science and Technology (VISTEC), Rayong, Thailand.
Maenpuen S; Department of Biochemistry and Center for Excellence in Protein and Enzyme Technology, Faculty of Science, Mahidol University, Bangkok, Thailand.
Munkajohnpong P; Department of Biochemistry, Faculty of Science, Burapha University, Chonburi, Thailand.
Chaiyen P; Department of Biochemistry and Center for Excellence in Protein and Enzyme Technology, Faculty of Science, Mahidol University, Bangkok, Thailand.
Tinikul R; Department of Biomolecular Science and Engineering, School of Biomolecular Science and Engineering, Vidyasirimedhi Institute of Science and Technology (VISTEC), Rayong, Thailand.

FEBS J ; 285(13): 2504-2519, 2018 07.

Article em En | MEDLINE | ID: mdl-29734522

Assuntos

Acinetobacter baumannii/enzimologia; Proteínas de Bactérias/metabolismo; Cisteína/metabolismo; Succinato-Semialdeído Desidrogenase/metabolismo; Acinetobacter baumannii/genética; Sequência de Aminoácidos; Substituição de Aminoácidos; Proteínas de Bactérias/química; Proteínas de Bactérias/genética; Biocatálise; Domínio Catalítico; Cristalografia por Raios X; Cisteína/química; Cisteína/genética; Concentração de Íons de Hidrogênio; Cinética; NADP/química; NADP/metabolismo; Homologia de Sequência de Aminoácidos; Especificidade por Substrato; Succinato-Semialdeído Desidrogenase/química; Succinato-Semialdeído Desidrogenase/genética; Ácido gama-Aminobutírico/análogos & derivados; Ácido gama-Aminobutírico/química; Ácido gama-Aminobutírico/metabolismo

Palavras-chave

NADP-cysteine adduct; nucleophilicity; pre-steady-state kinetics; reactive cysteine; succinic semialdehyde dehydrogenase

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas de Bactérias / Cisteína / Acinetobacter baumannii / Succinato-Semialdeído Desidrogenase Idioma: En Ano de publicação: 2018 Tipo de documento: Article

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