Detection of Antibiotic Resistance Determinants and Their Transmissibility among Clinically Isolated Carbapenem-Resistant Escherichia coli from South India.
Med Princ Pract
; 27(5): 428-435, 2018.
Article
em En
| MEDLINE
| ID: mdl-29739004
ABSTRACT
OBJECTIVES:
The aim of this study was to analyze the prevalence of the CTX-M, TEM, SHV, VIM, NDM, and OXA genes in carbapenemase-producing Escherichia coli and their transmissibility at a tertiary care hospital in south India. MATERIALS ANDMETHODS:
Twenty-one carbapenem-resistant E. coli (carbapenem-resistant Enterobacteriaceae; CRE) were collected from the Sri Sathya Sai Institute of Higher Medical Sciences (Puttaparthi India). Resistance to antibiotics was analyzed by Vitek-2, and the identity of the isolates was confirmed by 16S rDNA sequencing. RAPD and enterobacterial repetitive intergenic consensus (ERIC)-PCR were performed for molecular typing. Metallo-ß-lactamase production was confirmed by a double disc synergy test. The presence of the extended-spectrum ß-lactamases CTX-M, TEM, and SHV and of the carbapenemases NDM, VIM, and OXA was determined by PCR. Carbapenemase variants were further confirmed by sequencing. The transmissibility of the genes was tested by conjugation.RESULTS:
Twelve of the 21 (57%) carbapenem-resistant E. coli isolates were community acquired, indicating the spread of CRE in environmental samples. TEM and NDM-5 were found to be the major ß-lactamases produced by the pathogens. OXA-181 was found in 5 of the isolates. All 21 isolates were found to harbor more than one of the tested ß-lactamases, and all of the isolates were found to have the capacity to participate in conjugation; 15 of the transconjugants were found to have acquired the tested ß-lactamases, substantiating their ability to be transferred to other strains of bacteria.CONCLUSION:
Monitoring of community-acquired carbapenem-resistant bacteria is very important as the association of resistance determinants with mobile genetic elements would present a serious clinical challenge.Palavras-chave
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Base de dados:
MEDLINE
Assunto principal:
Farmacorresistência Bacteriana
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Escherichia coli
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Infecções por Escherichia coli
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Enterobacteriáceas Resistentes a Carbapenêmicos
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article