Microwave-Assisted Synthesis and Immunological Evaluation of Self-Assembling Peptide Vaccines.
Methods Mol Biol
; 1777: 249-259, 2018.
Article
em En
| MEDLINE
| ID: mdl-29744840
Self-assembling peptides spontaneously associate into functional supramolecular scaffolds, which have found numerous biomedical applications. These molecular assemblies have applications in nerve regeneration, wound healing, and both prophylactic and therapeutic vaccination. They can also be useful tools for proliferation assays, sustained culture of difficult cell lines, or activation of cell lines for immunoassays. This protocol will describe the basic peptide synthesis and purification of model self-assembling peptide immunogen and methods for vaccinating mice, collecting lymph nodes, and stimulating cells ex vivo.
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MEDLINE
Assunto principal:
Vacinas de Subunidades Antigênicas
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Multimerização Proteica
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Técnicas de Química Sintética
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Imunogenicidade da Vacina
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Micro-Ondas
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article