Postmortem role of calpain-11 in ostrich skeletal muscle.
Meat Sci
; 143: 147-152, 2018 Sep.
Article
em En
| MEDLINE
| ID: mdl-29751221
ABSTRACT
The postmortem calpain-11 role in ostrich muscle was investigated. Pairs of ostrich muscle (Iliotibialis cranialis) were excised from 32 ostrich carcasses in 3-h postmortem and randomly assigned into four treatments. The muscle was cut into 2.5-cm thick meat cores. The cores were incubated in 30â¯mM CaCl2, 30â¯mM EDTA, 90â¯mM NaCl, or control. The cores from the left-side carcasses were sampled after 0, 1, 2, and 3â¯days of incubation at 5⯰C, while the right-side meat cores were taken at 1-day and 3-day incubation for shear force measurements. The results showed that the decrease in unautolyzed and total activities of calpain-11, desmin content and shear force was more rapid in CaCl2-incubated samples than in control, NaCl- and EDTA-incubated samples. Thus, present results suggest that in the absence of calpain-1, calpain-11 with an extensive activation by adding exogenous Ca2+ could enhance the postmortem proteolysis and tenderization of ostrich muscle.
Palavras-chave
2-mercaptoethanol (PubChem CID: 1567); ACRYLAMIDE (PubChem CID: 6579); Alpha-Casein (PubChem CID: 134288); CALCIUM CHLORIDE (PubChem CID: 5284359); Calpain; EDTA (PubChem CID: 6049); EGTA (PubChem CID: 6207); Glycine (PubChem CID: 750); N, N'-Methylenediacrylamide (PubChem CID: 8041); Ostrich muscle; Postmortem proteolysis; Sodium dodecyl sulfate (PubChem CID: 3423265); Tenderization; Tris (PubChem CID: 6503)
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Calpaína
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Músculo Esquelético
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Proteínas Aviárias
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Armazenamento de Alimentos
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Carne
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article