Growth Factors Release From Concentrated Growth Factors: Effect of ß-Tricalcium Phosphate Addition.
J Craniofac Surg
; 29(8): 2291-2295, 2018 Nov.
Article
em En
| MEDLINE
| ID: mdl-29771832
ABSTRACT
BACKGROUND:
Platelet concentrates represent a new approach to improve tissue regeneration and can be used alone or together with autogenous bone, recombinant human growth factors, and/or other biomaterials, to enhance tissue regeneration. Among platelet concentrates, concentrated growth factors (CGFs) exhibit an interesting clinical and biotechnological application potential.OBJECTIVE:
The aim of this study was to evaluate the in vitro release of 4 growth factors (bone morphogenetic proteins [BMP] -2, BMP-7, transforming growth factor [TGF] -ß1, and insulin-like growth factor [IGF] -1) by the enzyme-linked immunosorbent assay (ELISA) technique, in CGFs mixed or not with ß-tricalcium phosphate (ß-TCP), using or not the Round-up device, at different times.METHODS:
CGFs were obtained from healthy volunteers, mixed or not with ß-TCP, using or not the Round-up device. The release of 4 growth factors from these CGFs was then measured at 5âhours, 1, 3, 6, and 8 days, using the ELISA assay.RESULTS:
Comparison of the results obtained with those achieved for CGFs alone showed that BMP2 and BMP-7 release, significantly increased in CGFs mixed with Round-up and ß-TCP, TGF-ß1 release was similar to CGFs alone, whereas IG-1 release was lower compared with CGFs alone.CONCLUSION:
The present data suggest that ß-TCP addition to CGF could enhance and improve tissue regeneration, especially bone regeneration, increasing the release of some growth factors that play an important role in osteogenesis.
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MEDLINE
Assunto principal:
Plaquetas
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Fosfatos de Cálcio
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Fator de Crescimento Insulin-Like I
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Fator de Crescimento Transformador beta1
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Proteína Morfogenética Óssea 2
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Proteína Morfogenética Óssea 7
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article