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Exogenous hydrogen sulfide ameliorates high glucose-induced myocardial injury & inflammation via the CIRP-MAPK signaling pathway in H9c2 cardiac cells.
Zhao, Hong-Lei; Wu, Bao-Quan; Luo, Ying; Zhang, Wen-Ying; Hao, Yun-Ling; Liang, Jin-Jie; Fang, Fang; Liu, Wei; Chen, Xie-Hui.
Afiliação
  • Zhao HL; Department of Geriatrics and Cardiovascular Medicine, Shenzhen Sun Yat-Sen Cardiovascular Hospital, Shenzhen 518112, PR China.
  • Wu BQ; Department of Geriatrics and Cardiovascular Medicine, Shenzhen Sun Yat-Sen Cardiovascular Hospital, Shenzhen 518112, PR China.
  • Luo Y; Department of Geriatrics and Cardiovascular Medicine, Shenzhen Sun Yat-Sen Cardiovascular Hospital, Shenzhen 518112, PR China.
  • Zhang WY; Department of Geriatrics and Cardiovascular Medicine, Shenzhen Sun Yat-Sen Cardiovascular Hospital, Shenzhen 518112, PR China.
  • Hao YL; Department of Geriatrics and Cardiovascular Medicine, Shenzhen Sun Yat-Sen Cardiovascular Hospital, Shenzhen 518112, PR China.
  • Liang JJ; Department of Geriatrics and Cardiovascular Medicine, Shenzhen Sun Yat-Sen Cardiovascular Hospital, Shenzhen 518112, PR China.
  • Fang F; Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, PR China.
  • Liu W; Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, PR China.
  • Chen XH; Department of Geriatrics and Cardiovascular Medicine, Shenzhen Sun Yat-Sen Cardiovascular Hospital, Shenzhen 518112, PR China. Electronic address: xhchen66@126.com.
Life Sci ; 208: 315-324, 2018 Sep 01.
Article em En | MEDLINE | ID: mdl-29857073
ABSTRACT

AIMS:

Hydrogen sulfide (H2S) is a novel signaling molecule with potent cytoprotective actions. In this study, we hypothesize that exogenous H2S may protect cardiac cells against high glucose (HG)-induced myocardial injury and inflammation with the involvement of the CIRP-MAPK signaling pathway. MAIN

METHODS:

H9c2 cardiac cells cultured under HG conditions were transfected with siRNA and different inhibitor for detecting the effects of sodium hydrogen sulfide (NaHS) (a H2S donor) on cell biological processes. The cardiac cell viability and LDH activity were determined by CCK-8 and LDH kit. ELISA was employed to measure the levels of inflammatory factors, while 2',7'-dichlorofluorescein diacetate (DCFH-DA) to evaluate reactive oxygen species (ROS). Mitochondrial membrane potential (MMP) was identified by rhodamine 123 staining. TUNEL staining and Hoechst 33258 staining were employed to observe cardiac cell apoptosis. Besides, we determined the expression of CIRP-MAPK signaling pathway- and apoptosis-related factors by protein immunoblot analysis. KEY

FINDINGS:

HG culturing induced toxicity, LDH, higher level of inflammatory factors, ROS, MMP, and apoptosis in cardiac cells, attenuated the viability of cardiac cells, and activated the CIRP-MAPK signaling pathway. Notably, CIRP silencing aggravated the above condition. H2S or blockade of the MAPK signaling pathway reversed the above conditions induced by HG.

SIGNIFICANCE:

The present study provides evidence for the protective effect of exogenous H2S on HG-induced myocardial injury and inflammation in H9c2 cardiac cells and suggests that the activation of CIRP-MAPK signaling pathway might be one of the mechanisms underlying the protective effect of H2S.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Traumatismo por Reperfusão Miocárdica / Proteínas de Ligação a RNA / Miócitos Cardíacos / Proteínas Quinases p38 Ativadas por Mitógeno / Proteínas e Peptídeos de Choque Frio / Glucose / Sulfeto de Hidrogênio / Inflamação Idioma: En Ano de publicação: 2018 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Traumatismo por Reperfusão Miocárdica / Proteínas de Ligação a RNA / Miócitos Cardíacos / Proteínas Quinases p38 Ativadas por Mitógeno / Proteínas e Peptídeos de Choque Frio / Glucose / Sulfeto de Hidrogênio / Inflamação Idioma: En Ano de publicação: 2018 Tipo de documento: Article