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BLT1-mediated O-GlcNAcylation is required for NOX2-dependent migration, exocytotic degranulation and IL-8 release of human mast cell induced by Trichomonas vaginalis-secreted LTB4.
Min, Arim; Lee, Young Ah; Kim, Kyeong Ah; Shin, Myeong Heon.
Afiliação
  • Min A; Department of Environmental Medical Biology, Institute of Tropical Medicine, South Korea; Yonsei University College of Medicine, Seoul 120-752, South Korea.
  • Lee YA; Department of Environmental Medical Biology, Institute of Tropical Medicine, South Korea; Yonsei University College of Medicine, Seoul 120-752, South Korea.
  • Kim KA; Department of Environmental Medical Biology, Institute of Tropical Medicine, South Korea.
  • Shin MH; Department of Environmental Medical Biology, Institute of Tropical Medicine, South Korea; Yonsei University College of Medicine, Seoul 120-752, South Korea. Electronic address: myeong@yuhs.ac.
Microbes Infect ; 20(6): 376-384, 2018.
Article em En | MEDLINE | ID: mdl-29859938
Trichomonas vaginalis is a sexually-transmitted protozoan parasite that causes vaginitis and cervicitis. Although mast cell activation is important for provoking tissue inflammation during infection with parasites, information regarding the signaling mechanisms in mast cell activation and T. vaginalis infection is limited. O-linked N-acetylglucosamine (O-GlcNAc) is a post-translational modification of serine and threonine residues that functions as a critical regulator of intracellular signaling, regulated by O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA). We investigated if O-GlcNAcylation was associated with mast cell activation induced by T. vaginalis-derived secretory products (TvSP). Modified TvSP collected from live trichomonads treated with the 5-lipooxygenase inhibitor AA861 inhibited migration of mast cells. This result suggested that mast cell migration was caused by stimulation of T. vaginalis-secreted leukotrienes. Using the BLT1 antagonist U75302 or BLT1 siRNA, we found that migration of mast cells was evoked via LTB4 receptor (BLT1). Furthermore, TvSP induced protein O-GlcNAcylation and OGT expression in HMC-1 cells, which was prevented by transfection with BLT1 siRNA. TvSP-induced migration, ROS generation, CD63 expression and IL-8 release were significantly suppressed by pretreatment with OGT inhibitor ST045849 or OGT siRNA. These results suggested that BLT1-mediated OGlcNAcylation was important for mast cell activation during trichomoniasis.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Acetilglucosamina / Trichomonas vaginalis / Receptores do Leucotrieno B4 / Leucotrieno B4 / NADPH Oxidase 2 / Mastócitos Idioma: En Ano de publicação: 2018 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Acetilglucosamina / Trichomonas vaginalis / Receptores do Leucotrieno B4 / Leucotrieno B4 / NADPH Oxidase 2 / Mastócitos Idioma: En Ano de publicação: 2018 Tipo de documento: Article