Optimization of the method for α-l-fucosidase, ß-d-galactosidase and ß-d-glucuronidase determination in serum from hemolyzed blood.
Adv Med Sci
; 63(2): 306-311, 2018 Sep.
Article
em En
| MEDLINE
| ID: mdl-29885630
ABSTRACT
PURPOSE:
Adaptation of the colorimetric method for the determination of ß-d-galactosidase, ß-d-glucuronidase and α-l-fucosidase activities in serums from hemolyzed blood, the material currently being discarded. MATERIALS ANDMETHODS:
The materials included serums from hemolyzed and non-hemolyzed blood, obtained from 26 healthy volunteers. The adaptation of the method involved precipitation of the proteins with trichloroacetic acid after incubating serums with substrates, but before determining the products of enzymatic reactions.RESULTS:
In serums from hemolyzed and non-hemolyzed blood of the same persons, we found high correlations among the results obtained using hemolyzed blood (with adapted) and non-hemolyzed blood (with non-adapted) methods.CONCLUSION:
We are able to determine the ß-d-galactosidase, ß-d-glucuronidase and α-l-fucosidase activities in serums from hemolyzed blood (with adapted) and non-hemolyzed blood (with non-adapted) methods, with the same accuracy and precision.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Beta-Galactosidase
/
Alfa-L-Fucosidase
/
Glucuronidase
/
Hemólise
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article