Modified Plasma-Thrombin Method of Cell Block Preparation for Fine-Needle Aspiration Biopsies in Resource-Limited Settings.
Am J Clin Pathol
; 150(2): 137-145, 2018 Jul 03.
Article
em En
| MEDLINE
| ID: mdl-29893770
ABSTRACT
OBJECTIVES:
The plasma-thrombin method is commonly used to make cell blocks from fine-needle aspiration (FNA) samples but requires centrifugation. We describe a modification to this method that does not require centrifugation for use in resource-limited settings.METHODS:
Pooled fresh plasma is aliquoted into 2-mL Eppendorf tubes and the FNA sample directly rinsed into the plasma. Two drops of reconstituted thrombin are added and gently mixed. A cell clot is transferred to a tissue bag, fixed in formalin, and processed. This method was applied to FNA samples from 44 patients presenting to the Mbarara University of Science and Technology FNA clinic.RESULTS:
The cell blocks were less cellular than the smears but contained adequate material to confirm morphologic impression or perform immunocytochemistry in 36 of 44 cases (82% adequacy rate).CONCLUSIONS:
The modified plasma-thrombin method is a reliable cell block preparation method that can be easily applied in resource-limited settings.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Técnicas de Preparação Histocitológica
/
Biópsia por Agulha Fina
/
Neoplasias
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article