Duplication of a Single Strand in a ß-Sheet Can Produce a New Switching Function in a Photosensory Protein.
Biochemistry
; 57(28): 4093-4104, 2018 07 17.
Article
em En
| MEDLINE
| ID: mdl-29897240
ABSTRACT
Duplication of a single ß-strand that forms part of a ß-sheet in photoactive yellow protein (PYP) was found to produce two approximately isoenergetic protein conformations, in which either the first or the second copy of the duplicated ß-strand participates in the ß-sheet. Whereas one conformation (big-loop) is more stable at equilibrium in the dark, the other conformation (long-tail) is populated after recovery from blue light irradiation. By appending a recognition motif (E-helix) to the C-terminus of the protein, we show that ß-strand duplication, and the resulting possibility of ß-strand slippage, can lead to a new switchable protein-protein interaction. We suggest that ß-strand duplication may be a general means of introducing two-state switching activity into protein structures.
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1
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Bactérias
/
Fotorreceptores Microbianos
/
Halorhodospira halophila
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article