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Na+ extrusion from the cytosol and tissue-specific Na+ sequestration in roots confer differential salt stress tolerance between durum and bread wheat.
Wu, Honghong; Shabala, Lana; Azzarello, Elisa; Huang, Yuqing; Pandolfi, Camilla; Su, Nana; Wu, Qi; Cai, Shengguan; Bazihizina, Nadia; Wang, Lu; Zhou, Meixue; Mancuso, Stefano; Chen, Zhonghua; Shabala, Sergey.
Afiliação
  • Wu H; School of Land and Food, University of Tasmania, Private Bag, Hobart, Tasmania, Australia.
  • Shabala L; School of Land and Food, University of Tasmania, Private Bag, Hobart, Tasmania, Australia.
  • Azzarello E; Department of Horticulture, University of Florence, Sesto Fiorentino, Italy.
  • Huang Y; School of Science and Health, Hawkesbury Institute for the Environment, Western Sydney University, Penrith, NSW, Australia.
  • Pandolfi C; Department of Horticulture, University of Florence, Sesto Fiorentino, Italy.
  • Su N; School of Land and Food, University of Tasmania, Private Bag, Hobart, Tasmania, Australia.
  • Wu Q; School of Land and Food, University of Tasmania, Private Bag, Hobart, Tasmania, Australia.
  • Cai S; School of Science and Health, Hawkesbury Institute for the Environment, Western Sydney University, Penrith, NSW, Australia.
  • Bazihizina N; School of Land and Food, University of Tasmania, Private Bag, Hobart, Tasmania, Australia.
  • Wang L; Department of Horticulture, University of Florence, Sesto Fiorentino, Italy.
  • Zhou M; School of Natural Sciences, University of Tasmania, Private Bag 55, Hobart, Tasmania, Australia.
  • Mancuso S; School of Land and Food, University of Tasmania, Private Bag, Hobart, Tasmania, Australia.
  • Chen Z; Department of Horticulture, University of Florence, Sesto Fiorentino, Italy.
  • Shabala S; School of Science and Health, Hawkesbury Institute for the Environment, Western Sydney University, Penrith, NSW, Australia.
J Exp Bot ; 69(16): 3987-4001, 2018 07 18.
Article em En | MEDLINE | ID: mdl-29897491
ABSTRACT
The progress in plant breeding for salinity stress tolerance is handicapped by the lack of understanding of the specificity of salt stress signalling and adaptation at the cellular and tissue levels. In this study, we used electrophysiological, fluorescence imaging, and real-time quantitative PCR tools to elucidate the essentiality of the cytosolic Na+ extrusion in functionally different root zones (elongation, meristem, and mature) in a large number of bread and durum wheat accessions. We show that the difference in the root's ability for vacuolar Na+ sequestration in the mature zone may explain differential salinity stress tolerance between salt-sensitive durum and salt-tolerant bread wheat species. Bread wheat genotypes also had on average 30% higher capacity for net Na+ efflux from the root elongation zone, providing the first direct evidence for the essentiality of the root salt exclusion trait at the cellular level. At the same time, cytosolic Na+ accumulation in the root meristem was significantly higher in bread wheat, leading to the suggestion that this tissue may harbour a putative salt sensor. This hypothesis was then tested by investigating patterns of Na+ distribution and the relative expression level of several key genes related to Na+ transport in leaves in plants with intact roots and in those in which the root meristems were removed. We show that tampering with this sensing mechanism has resulted in a salt-sensitive phenotype, largely due to compromising the plant's ability to sequester Na+ in mesophyll cell vacuoles. The implications of these findings for plant breeding for salinity stress tolerance are discussed.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Sódio / Triticum / Raízes de Plantas / Citosol / Tolerância ao Sal / Estresse Salino Idioma: En Ano de publicação: 2018 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Sódio / Triticum / Raízes de Plantas / Citosol / Tolerância ao Sal / Estresse Salino Idioma: En Ano de publicação: 2018 Tipo de documento: Article