Long noncoding RNA AFAP1AS1 enhances cell proliferation and invasion in osteosarcoma through regulating miR46955p/TCF4ßcatenin signaling.
Mol Med Rep
; 18(2): 1616-1622, 2018 Aug.
Article
em En
| MEDLINE
| ID: mdl-29901121
ABSTRACT
Long noncoding RNA AFAP1AS1 has been shown to promote tumor progression in several human cancer types, such as thyroid cancer, tongue squamous cell carcinoma and lung cancer. However, the role of AFAP1AS1 in osteosarcoma (OS) has not been investigated. In the present study, the expression of AFAP1AS1 was significantly upregulated in OS tissues and cell lines. Moreover, AFAP1AS1 expression was negatively correlated with OS patient prognosis. Besides, AFAP1AS1 knockdown significantly inhibited the proliferation and invasion of OS cells in vitro. Furthermore, in vivo xenograft experiments indicated that AFAP1AS1 depletion delayed tumor growth. Regarding the underlying mechanism, AFAP1AS1 served as a sponge to repress the level of microRNA (miR)46955p, which targeted transcription factor (TCF)4, a pivot effector of Wnt/ßcatenin signaling pathway. It was demonstrated that overexpression of AFAP1AS1 inhibited the expression of miR46955p, while miR46955p overexpression decreased TCF4 expression and reduced activation of Wnt/ßcatenin pathway. Through rescue assays, it was demonstrated that restoration of TCF4 expression reversed the effects of AFAP1AS1 knockdown or miR46955p overexpression on OS cells. Taken together, these findings demonstrated that the AFAP1AS1/miR46955p/TCF4ßcatenin axis played an important role in OS progression.
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Base de dados:
MEDLINE
Assunto principal:
Neoplasias Ósseas
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Osteossarcoma
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Regulação Neoplásica da Expressão Gênica
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MicroRNAs
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Beta Catenina
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RNA Longo não Codificante
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Fator de Transcrição 4
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article