Evaluation of T cells in blood after a short gluten challenge for coeliac disease diagnosis.
Dig Liver Dis
; 50(11): 1183-1188, 2018 11.
Article
em En
| MEDLINE
| ID: mdl-29903545
ABSTRACT
BACKGROUND AND AIM:
To diagnose coeliac disease (CD) in individuals on a gluten free diet (GFD), we aimed to assess the utility of detecting activated γδ and CD8â¯T cells expressing gut-homing receptors after a short gluten challenge.METHODS:
We studied 15 CD patients and 35 non-CD controls, all exposed to three days of gluten when following a GFD. Peripheral blood was collected before and six days after starting gluten consumption, and the expression of CD103, ß7 and CD38 in γδ and CD8â¯T cells was assessed by flow cytometry. Determination of IFN-γ and IP-10 was performed by means of ELISPOT and/or Luminex technology.RESULTS:
We observed both γδ and CD8â¯T cells coexpressing CD103, ß7hi and CD38 in every patient with CD on day six, but only in one control. The studied CD8â¯T subpopulation was easier to detect than the γδ subpopulation. Increased IFN-γ and IP-10 levels after challenge were observed in patients with CD, but not in controls.CONCLUSION:
A short three-day gluten challenge elicits the activation of CD103+ ß7hi CD8+ T cells in CD. These cells can be detected by flow cytometry in peripheral blood, opening new possibilities for CD diagnosis in individuals on a GFD.Palavras-chave
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Base de dados:
MEDLINE
Assunto principal:
Doença Celíaca
/
Linfócitos T CD8-Positivos
/
Glutens
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article