Structural and histological differences between connective tissue grafts harvested from the lateral palatal mucosa or from the tuberosity area.
Clin Oral Investig
; 23(2): 957-964, 2019 Feb.
Article
em En
| MEDLINE
| ID: mdl-29915931
ABSTRACT
Tuberosity grafts had a greater percentage of lamina propria and lower percentage of submucosa when compared to lateral palate grafts. OBJECTIVE:
The study aims to understand the differences in the structural composition of soft tissue autografts harvested from the lateral palate or the tuberosity. MATERIAL ANDMETHODS:
Patients were randomly allocated to receive autografts harvested either from palatal or tuberosity sites to augment horizontal volume deficiencies around single-tooth implants. Tissue biopsies were analyzed for histological and histo-morphometric analysis. Picro-sirius red stain was used to evaluate collagen 1 and 3. Also, immuno-histochemical analysis was performed against MMP1, MMP2, cytokeratin-10, cytokeratin-13, and lysine hydroxylase-2.RESULTS:
Twenty specimens were harvested from 9 subjects in the lateral palate group (PG) and 11 subjects in the tuberosity group (TG). The percentage of lamina propria represented 51.08% in the PG group and 72.79% in the TG group, while the area of submucosa was minimal in the TG group representing 4.89% of the total sample vs 25.75% in the PG. The total area of COL-1 and 3 in the TG was 1.19 ± 0.57 and 0.72 ± 0.44 mm2, respectively, while in the PG, the corresponding values were 1.4 ± 0.7 and 1.04 ± 0.5 mm2. The immuno-histochemical analysis generally showed a higher expression of LLH-2, MMP2, CYT-10, and CYT-13 in the TG when compared with the PG.CONCLUSION:
Tuberosity grafts had a greater percentage of lamina propria and lower percentage of submucosa. The collagen content in the lamina propria was similar for both groups while the immuno-histochemical profile showed differences in the antibody expression of the epithelial cells. CLINICAL RELEVANCE Tuberosity grafts had more lamina propria and less submocusa, which may be beneficial for volume augmentation.Palavras-chave
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Base de dados:
MEDLINE
Assunto principal:
Palato
/
Tecido Conjuntivo
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Implantes Dentários para Um Único Dente
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Retração Gengival
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article