Ultrasonic Control of Neural Activity through Activation of the Mechanosensitive Channel MscL.
Nano Lett
; 18(7): 4148-4155, 2018 07 11.
Article
em En
| MEDLINE
| ID: mdl-29916253
ABSTRACT
Externally controlling the excitation of a neuronal subset through ion channels activation can modulate the firing pattern of an entire neural circuit in vivo. As nanovalves in the cell membrane, ion channels can be opened by light (optogenetics) or ultrasonic (sonogenetics) means. A thoroughly analyzed force sensor is the Escherichia coli mechano sensitive channel of large conductance (MscL). Here we expressed MscL in rat hippocampal neurons in a primary culture and showed that it could be activated by low-pressure ultrasound pulses. The gain-of-function mutation, I92L, sensitized MscL's sonic response, triggering action potentials at a peak negative pressure as low as 0.25 MPa. Further, the I92L MscL reliably elicited individual spikes by timed brief pulses, making excitation programmable. Because MscL opens to tension in the lipid bilayer, requiring no other proteins or ligands, it could be developed into a general noninvasive sonogenetic tool to manipulate the activities of neurons or other cells and potential nanodevices.
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MEDLINE
Assunto principal:
Membrana Celular
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Proteínas de Escherichia coli
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Canais Iônicos
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Neurônios
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article