Mediator and RNA polymerase II clusters associate in transcription-dependent condensates.
Science
; 361(6400): 412-415, 2018 07 27.
Article
em En
| MEDLINE
| ID: mdl-29930094
ABSTRACT
Models of gene control have emerged from genetic and biochemical studies, with limited consideration of the spatial organization and dynamics of key components in living cells. We used live-cell superresolution and light-sheet imaging to study the organization and dynamics of the Mediator coactivator and RNA polymerase II (Pol II) directly. Mediator and Pol II each form small transient and large stable clusters in living embryonic stem cells. Mediator and Pol II are colocalized in the stable clusters, which associate with chromatin, have properties of phase-separated condensates, and are sensitive to transcriptional inhibitors. We suggest that large clusters of Mediator, recruited by transcription factors at large or clustered enhancer elements, interact with large Pol II clusters in transcriptional condensates in vivo.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Fatores de Transcrição
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Transcrição Gênica
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RNA Polimerase II
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Regulação da Expressão Gênica
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Complexo Mediador
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article