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HPTLC fingerprint profile analysis of cocoa proanthocyanidins depending on origin and genotype.
Pedan, Vasilisa; Weber, Carlo; Do, Tiên; Fischer, Norbert; Reich, Eike; Rohn, Sascha.
Afiliação
  • Pedan V; Zurich University of Applied Sciences, Life Sciences und Facility Management, 8820 Wädenswil, Switzerland. Electronic address: vasilisa.pedan@zhaw.ch.
  • Weber C; Zurich University of Applied Sciences, Life Sciences und Facility Management, 8820 Wädenswil, Switzerland.
  • Do T; CAMAG Laboratory, Sonnenmattstrasse 41, 4132 Muttenz, Switzerland.
  • Fischer N; Zurich University of Applied Sciences, Life Sciences und Facility Management, 8820 Wädenswil, Switzerland.
  • Reich E; CAMAG Laboratory, Sonnenmattstrasse 41, 4132 Muttenz, Switzerland.
  • Rohn S; University of Hamburg, Hamburg School of Food Science, Institute of Food Chemistry, Grindelallee 117, 20146 Hamburg, Germany.
Food Chem ; 267: 277-287, 2018 Nov 30.
Article em En | MEDLINE | ID: mdl-29934168
Cocoa beans are rich in bioactive phytochemicals such as alkaloids, anthocyanins, as well as monomeric and oligomeric flavan-3-ols. A high performance thin layer chromatography (HPTLC) method was developed for a fast analysis of a fingerprint of bioactive compounds present in cocoa beans depending on genotype and origin. Evaluation was performed using HPTLC followed by densitometry. The best separation for a fingerprint consisting of eight phenolic compounds as markers was achieved on silica gel 60 F254 HPTLC plates with a solvent mixture of ethyl formate, formic acid, water, toluene 30/4/3/1.5 (v/v/v/v). Staining with Fast Blue Salt B enabled visualization and quantitative evaluation. Compounds of interest were confirmed by eluting zones using a TLC-MS interface. LODs were ≤ 100ng/zone for all compounds. The fingerprints obtained are useful for quality control of cocoa beans allowing their differentiation according to cocoa or chocolate sorts, varieties, and origins.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Cacau / Cromatografia em Camada Fina / Proantocianidinas Idioma: En Ano de publicação: 2018 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Cacau / Cromatografia em Camada Fina / Proantocianidinas Idioma: En Ano de publicação: 2018 Tipo de documento: Article