Evaluation of optical imaging agents in a fluorescence-guided surgical model of head and neck cancer.
Surg Oncol
; 27(2): 225-230, 2018 Jun.
Article
em En
| MEDLINE
| ID: mdl-29937175
ABSTRACT
BACKGROUND:
Tumor proliferation often occurs from pathologic receptor upregulation. These receptors provide unique targets for near-infrared (NIR) probes that have fluorescence-guided surgery (FGS) applications. We demonstrate the use of three smart-targeted probes in a model of head and neck squamous cell carcinoma.METHODS:
A dose escalation study was performed using IntegriSense750, ProSense750EX, and ProSense750FAST in mice (nâ¯=â¯5) bearing luciferase-positive SCC-1 flank xenograft tumors. Whole body fluorescence imaging was performed serially after intravenous injection using commercially available open-field (LUNA, Novadaq, Canada) and closed-field NIR systems (Pearl, LI-COR, Lincoln, NE). An ex vivo, whole-body biodistribution was conducted. Lastly, FGS was performed with IntegriSense750 to demonstrate orthotopic and metastatic disease localization.RESULTS:
Disease fluorescence delineation was assessed by tumor-to-background fluorescence ratios (TBR). Peak TBR values were 3.3 for 1â¯nmol ProSense750EX, 5.5 for 6â¯nmol ProSense750FAST, and 10.8 for 4â¯nmol IntegriSense750â¯at 5.5, 3, and 4â¯d post administration, respectively. Agent utility is unique ProSense750FAST provides sufficient contrast quickly (TBR 1.5, 3â¯h) while IntegriSense750 produces strong (TBR 10.8) contrast with extended administration-to-resection time (96â¯h). IntegriSense750 correctly identified all diseased nodes in situ during exploratory surgeries. Ex vivo, whole-body biodistribution was assessed by tumor-to-tissue fluorescence ratios (TTR). Agents provided sufficient fluorescence contrast to discriminate disease from background, TTR>1. IntegriSense750 was most robust in neural tissue (TTR 64) while ProSense750EX was superior localizing disease against lung tissue (TBR 13).CONCLUSION:
All three agents appear effective for FGS.Palavras-chave
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Base de dados:
MEDLINE
Assunto principal:
Carcinoma de Células Escamosas
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Cirurgia Assistida por Computador
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Imagem Óptica
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Corantes Fluorescentes
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Neoplasias de Cabeça e Pescoço
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Modelos Anatômicos
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article