DeTiN: overcoming tumor-in-normal contamination.
Nat Methods
; 15(7): 531-534, 2018 07.
Article
em En
| MEDLINE
| ID: mdl-29941871
ABSTRACT
Comparison of sequencing data from a tumor sample with data from a matched germline control is a key step for accurate detection of somatic mutations. Detection sensitivity for somatic variants is greatly reduced when the matched normal sample is contaminated with tumor cells. To overcome this limitation, we developed deTiN, a method that estimates the tumor-in-normal (TiN) contamination level and, in cases affected by contamination, improves sensitivity by reclassifying initially discarded variants as somatic.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Análise de Sequência de DNA
/
Sequenciamento de Nucleotídeos em Larga Escala
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Neoplasias
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article