Recombinant club cell protein 16 (CC16) ameliorates cigarette smokeinduced lung inflammation in a murine disease model of COPD.
Mol Med Rep
; 18(2): 2198-2206, 2018 Aug.
Article
em En
| MEDLINE
| ID: mdl-29956762
ABSTRACT
Club cell protein (CC16) is expressed primarily by club cells possesses antiinflammatory properties and is located in the bronchiolar epithelium. Previous studies have demonstrated that CC16 deficiency is associated with the progression of chronic obstructive pulmonary disease (COPD). In the present study, the therapeutic effects of recombinant rat CC16 protein in mice with COPD were examined and the underlying mechanisms investigated. A total of 30 adult male C57/BL6 mice were randomly divided into three groups (10 mice/group). A mouse COPD model was generated by exposing 20 mice to cigarette smoke (CS) for 24 weeks. A total of 10 mice were treated intranasally with rCC16 (2.5 µg/g body weight) and control mice were exposed to normal room air. Results indicated that rCC16 treatment ameliorated pathological damage in the lungs and reduced the production of tumor necrosis factor (TNF)α, interleukin (IL)6 and IL8, which were induced by CS exposure. After rCC16 administration, endogenous CC16 was upregulated and the body weight of COPD mice was increased, whereas the opposite was observed in CSexposed mice. Additionally, rCC16 treatment inhibited the DNA binding of NFκB/p65 in lung tissues and reduced nuclear translocation of NFκB/p65 in BALF and epithelial cells. Moreover, rCC16 treatment lead to a decrease in the total number of BALF cells, including macrophages, which was elevated in COPD mice. In conclusion, the present results demonstrate that rCC16 has therapeutic effects on COPD by downregulating proinflammatory factors via the NFκB pathway.
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Base de dados:
MEDLINE
Assunto principal:
Pneumonia
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Uteroglobina
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Transdução de Sinais
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Mucosa Respiratória
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Doença Pulmonar Obstrutiva Crônica
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Fumar Cigarros
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article