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Ischemic Model of Heart Failure in Rats and Mice.
Chen, Jiqiu; Ceholski, Delaine K; Turnbull, Irene C; Liang, Lifan; Hajjar, Roger J.
Afiliação
  • Chen J; Cardiovascular Research Center, Icahn School of Medicine at Mount Sinai, New York, NY, USA. jiqiu.chen@mssm.edu.
  • Ceholski DK; Cardiovascular Research Center, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
  • Turnbull IC; Cardiovascular Research Center, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
  • Liang L; Cardiovascular Research Center, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
  • Hajjar RJ; Cardiovascular Research Center, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
Methods Mol Biol ; 1816: 175-182, 2018.
Article em En | MEDLINE | ID: mdl-29987819
ABSTRACT
Temporary or permanent left coronary artery (LCA) ligation is the most widely used model of heart failure. In the present protocol, we describe the materials necessary for the procedure, key steps of the LCA ligation, triphenyl tetrazolium chloride (TTC) staining, and calculation of myocardial infarction (MI) size after ischemia-reperfusion (I/R) injury (30 min/24 h) in rats and mice. We discuss precautions and tips regarding the operation before and after surgery, both in vivo and ex vivo. The aim of this chapter is to describe the details of LCA surgery and provide recommendations for current and future surgical operators.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Isquemia Miocárdica / Modelos Animais de Doenças / Insuficiência Cardíaca / Miocárdio Idioma: En Ano de publicação: 2018 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Isquemia Miocárdica / Modelos Animais de Doenças / Insuficiência Cardíaca / Miocárdio Idioma: En Ano de publicação: 2018 Tipo de documento: Article