Fluorescence techniques in developmental biology.
J Biosci
; 43(3): 541-553, 2018 Jul.
Article
em En
| MEDLINE
| ID: mdl-30002271
ABSTRACT
Advanced fluorescence techniques, commonly known as the F-techniques, measure the kinetics and the interactions of biomolecules with high sensitivity and spatiotemporal resolution. Applications of the F-techniques, which were initially limited to cells, were further extended to study in vivo protein organization and dynamics in whole organisms. The integration of F-techniques with multi-photon microscopy and light-sheet microscopy widened their applications in the field of developmental biology. It became possible to penetrate the thick tissues of living organisms and obtain good signal-to-noise ratio with reduced photo-induced toxicity. In this review, we discuss the principle and the applications of the three most commonly used F-techniques in developmental biology Fluorescence Recovery After Photo-bleaching (FRAP), Fo¨ rster Resonance Energy Transfer (FRET), and Fluorescence Correlation and Cross-Correlation Spectroscopy (FCS and FCCS).
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Base de dados:
MEDLINE
Assunto principal:
Espectrometria de Fluorescência
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Xenopus laevis
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Peixe-Zebra
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Caenorhabditis elegans
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Drosophila melanogaster
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Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article