Reengineering the ligand sensitivity of the broadly tuned human bitter taste receptor TAS2R14.

Nowak, Stefanie; Di Pizio, Antonella; Levit, Anat; Niv, Masha Y; Meyerhof, Wolfgang; Behrens, Maik

Nowak, Stefanie; Di Pizio, Antonella; Levit, Anat; Niv, Masha Y; Meyerhof, Wolfgang; Behrens, Maik.

Afiliação

Nowak S; German Institute of Human Nutrition Potsdam-Rehbruecke, Dept. Molecular Genetics, 14558 Nuthetal, Germany.
Di Pizio A; The Institute of Biochemistry, Food and Nutrition, The Robert H Smith Faculty of Agriculture, Food and Environment, The Hebrew University, 76100 Rehovot, Israel; The Fritz Haber Center for Molecular Dynamics, The Hebrew University, Jerusalem 91904, Israel.
Levit A; The Institute of Biochemistry, Food and Nutrition, The Robert H Smith Faculty of Agriculture, Food and Environment, The Hebrew University, 76100 Rehovot, Israel; The Fritz Haber Center for Molecular Dynamics, The Hebrew University, Jerusalem 91904, Israel; Department of Pharmaceutical Chemistry, Uni
Niv MY; The Institute of Biochemistry, Food and Nutrition, The Robert H Smith Faculty of Agriculture, Food and Environment, The Hebrew University, 76100 Rehovot, Israel; The Fritz Haber Center for Molecular Dynamics, The Hebrew University, Jerusalem 91904, Israel.
Meyerhof W; German Institute of Human Nutrition Potsdam-Rehbruecke, Dept. Molecular Genetics, 14558 Nuthetal, Germany; Center for Integrative Physiology and Molecular Medicine, Saarland University, 66421 Homburg, Germany.
Behrens M; German Institute of Human Nutrition Potsdam-Rehbruecke, Dept. Molecular Genetics, 14558 Nuthetal, Germany; Leibniz-Institute for Food Systems Biology, Technical University of Munich, 85354 Freising, Germany. Electronic address: m.behrens.leibniz-lsb@tum.de.

Biochim Biophys Acta Gen Subj ; 1862(10): 2162-2173, 2018 10.

Article em En | MEDLINE | ID: mdl-30009876

Assuntos

Ácidos Aristolóquicos/metabolismo; Monoterpenos/metabolismo; Picrotoxina/análogos & derivados; Receptores Acoplados a Proteínas G/química; Receptores Acoplados a Proteínas G/metabolismo; Paladar/fisiologia; Sequência de Aminoácidos; Ácidos Aristolóquicos/química; Monoterpenos Bicíclicos; Sítios de Ligação; Humanos; Ligantes; Modelos Moleculares; Simulação de Acoplamento Molecular; Monoterpenos/química; Mutagênese Sítio-Dirigida; Mutação; Picrotoxina/química; Picrotoxina/metabolismo; Ligação Proteica; Conformação Proteica; Engenharia de Proteínas; Receptores Acoplados a Proteínas G/agonistas; Receptores Acoplados a Proteínas G/genética; Sesterterpenos

Palavras-chave

Bitter taste receptor; Calcium imaging; G protein-coupled receptor (GPCR); In vitro mutagenesis; Molecular modeling; Structure-function relationship

Texto completo

Imprimir

XML

PubMed Links

Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Picrotoxina / Paladar / Monoterpenos / Ácidos Aristolóquicos / Receptores Acoplados a Proteínas G Idioma: En Ano de publicação: 2018 Tipo de documento: Article

Texto completo

Imprimir

XML

PubMed Links

Buscar no Google