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Rapid selection of astaxanthin-hyperproducing Haematococcus mutant via azide-based colorimetric assay combined with oil-based astaxanthin extraction.
Hong, Min Eui; Choi, Hong Il; Kwak, Ho Seok; Hwang, Sung-Won; Sung, Young Joon; Chang, Won Seok; Sim, Sang Jun.
Afiliação
  • Hong ME; Department of Chemical and Biological Engineering, Korea University, 145, Anam-ro, Seongbuk-gu, Seoul 02841, South Korea.
  • Choi HI; Department of Chemical and Biological Engineering, Korea University, 145, Anam-ro, Seongbuk-gu, Seoul 02841, South Korea.
  • Kwak HS; Department of Food Science and Engineering, Dongyang Mirae University, 445, Gyeongin-ro, Guro-gu, Seoul 08221, South Korea.
  • Hwang SW; Department of Chemical and Biological Engineering, Korea University, 145, Anam-ro, Seongbuk-gu, Seoul 02841, South Korea.
  • Sung YJ; Department of Chemical and Biological Engineering, Korea University, 145, Anam-ro, Seongbuk-gu, Seoul 02841, South Korea.
  • Chang WS; Research Institute, Korea District Heating Corp., 92, Gigok-ro, Giheung-gu, Yongin-si, Gyeonggi-do 17099, South Korea.
  • Sim SJ; Department of Chemical and Biological Engineering, Korea University, 145, Anam-ro, Seongbuk-gu, Seoul 02841, South Korea. Electronic address: simsj@korea.ac.kr.
Bioresour Technol ; 267: 175-181, 2018 Nov.
Article em En | MEDLINE | ID: mdl-30014996
The aim of this work was to develop a new approach for simple and high-throughput selection of astaxanthin-hyperproducing Haematococcus mutants through a sequential combination method of azide-based colorimetric assessment and oil-based astaxanthin quantification. Randomly mutagenized cells were spotted on solid culture medium containing 50 µM of sodium azide to accelerate the biosynthesis of astaxanthin. After 3 days, highly-induced mutants were preliminarily isolated by visual inspection and their astaxanthin accumulations were rapidly quantified by soybean oil-based extraction method. On the whole, the selected mutants showed reduced vegetative growth rates but eventually exhibited higher astaxanthin productions than the parental strain owing to their improved inductive growths. Among them, M13 showed 174.7 ±â€¯5.69 mg L-1 of the highest astaxanthin production, which is 1.59-times higher than that of wild-type. This wide-scope screening method expedites both upstream and downstream astaxanthin quantification, making it a useful tool for isolating microalgae with high astaxanthin production.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Clorófitas Idioma: En Ano de publicação: 2018 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Clorófitas Idioma: En Ano de publicação: 2018 Tipo de documento: Article