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Multi-drug resistant Pseudomonas aeruginosa nosocomial strains: Molecular epidemiology and evolution.
Angeletti, Silvia; Cella, Eleonora; Prosperi, Mattia; Spoto, Silvia; Fogolari, Marta; De Florio, Lucia; Antonelli, Francesca; Dedej, Etleva; De Flora, Cecilia; Ferraro, Elisabetta; Incalzi, Raffaele Antonelli; Coppola, Roberto; Dicuonzo, Giordano; Francescato, Fabio; Pascarella, Stefano; Ciccozzi, Massimo.
Afiliação
  • Angeletti S; Unit of Clinical Laboratory Science, University Campus Bio-Medico of Rome, Italy. Electronic address: s.angeletti@unicampus.it.
  • Cella E; Unit of Medical Statistics and Molecular Epidemiology, University Campus Bio-Medico of Rome, Italy.
  • Prosperi M; Department of Epidemiology, College of Public Health and Health Professions & College of Medicine University of Florida, Gainesville, FL, USA.
  • Spoto S; Internal Medicine Department, University Campus Bio-Medico of Rome, Italy.
  • Fogolari M; Unit of Clinical Laboratory Science, University Campus Bio-Medico of Rome, Italy.
  • De Florio L; Unit of Clinical Laboratory Science, University Campus Bio-Medico of Rome, Italy.
  • Antonelli F; Unit of Clinical Laboratory Science, University Campus Bio-Medico of Rome, Italy.
  • Dedej E; Unit of Clinical Laboratory Science, University Campus Bio-Medico of Rome, Italy.
  • De Flora C; Unit of Clinical Laboratory Science, University Campus Bio-Medico of Rome, Italy.
  • Ferraro E; Unit of Clinical Laboratory Science, University Campus Bio-Medico of Rome, Italy.
  • Incalzi RA; Unit of Geriatrics, Department of Medicine, University Campus Bio-Medico of Rome, Italy.
  • Coppola R; Department of Surgery, University Campus Bio-Medico of Rome, Italy.
  • Dicuonzo G; Infection Control Committee, University Campus Bio-Medico of Rome, Italy.
  • Francescato F; Department of Biochemical Science "A. Rossi Fanelli", Sapienza University, Rome, Italy.
  • Pascarella S; Department of Biochemical Science "A. Rossi Fanelli", Sapienza University, Rome, Italy.
  • Ciccozzi M; Unit of Medical Statistics and Molecular Epidemiology, University Campus Bio-Medico of Rome, Italy.
Microb Pathog ; 123: 233-241, 2018 Oct.
Article em En | MEDLINE | ID: mdl-30031889
Pseudomonas aeruginosa causes a wide variety of nosocomial infections. In the study, phylogenetic, selective pressure analysis and homology modelling were applied to oprD efflux pump gene with the aim to characterize multi-drug resistant strains circulating in the nosocomial setting, their transmission dynamics and ongoing evolution. One hundred ninety-three consecutive inpatients with Pseudomonas aeruginosa infection were enrolled at the University Campus Bio-Medico of Rome, between January 2015 and December 2016. oprD gene was sequenced in 20 nosocomial multi-drug resistant P. aeruginosa strains. Phylogeographic, selective pressure, residue conservation analysis and homology modelling were performed. Clinical epidemiological data were extracted from patient medical records. Multi-drug resistant strains accounted for the 36% of total strains and were responsible of 20 cases of nosocomial infections. P. aeruginosa infections occurred prevalently in the West area, especially at the location IIIW and in the Geriatric ward. The time of the most recent common ancestor indicated that strains could have been introduced in the hospital since the end of the year 2009 with the most probable location in general surgery ward. By selective pressure analysis, 29 positions under diversifying selection have been identified and mapped onto the OprD model. Most of the observed residue substitutions are predicted to be destabilizing and some of them occurred in the Loops 2 and 3 that are involved in solute selection and carbapenem susceptibility. The molecular and evolutionary analysis of Multi-drug resistant strains circulating in the nosocomial setting may provide useful insights into the epidemiology and the mechanisms leading to resistance, contributing to infection control improvement.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Filogenia / Pseudomonas aeruginosa / Infecção Hospitalar / Porinas / Epidemiologia Molecular / Farmacorresistência Bacteriana Múltipla Idioma: En Ano de publicação: 2018 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Filogenia / Pseudomonas aeruginosa / Infecção Hospitalar / Porinas / Epidemiologia Molecular / Farmacorresistência Bacteriana Múltipla Idioma: En Ano de publicação: 2018 Tipo de documento: Article