hnRNPs Interacting with mRNA Localization Motifs Define Axonal RNA Regulons.
Mol Cell Proteomics
; 17(11): 2091-2106, 2018 11.
Article
em En
| MEDLINE
| ID: mdl-30038033
ABSTRACT
mRNA translation in axons enables neurons to introduce new proteins at sites distant from their cell body. mRNA-protein interactions drive this post-transcriptional regulation, yet knowledge of RNA binding proteins (RBP) in axons is limited. Here we used proteomics to identify RBPs interacting with the axonal localizing motifs of Nrn1, Hmgb1, Actb, and Gap43 mRNAs, revealing many novel RBPs in axons. Interestingly, no RBP is shared between all four RNA motifs, suggesting graded and overlapping specificities of RBP-mRNA pairings. A systematic assessment of axonal mRNAs interacting with hnRNP H1, hnRNP F, and hnRNP K, proteins that bound with high specificity to Nrn1 and Hmgb1, revealed that axonal mRNAs segregate into axon growth-associated RNA regulons based on hnRNP interactions. Axotomy increases axonal transport of hnRNPs H1, F, and K, depletion of these hnRNPs decreases axon growth and reduces axonal mRNA levels and axonal protein synthesis. Thus, subcellular hnRNP-interacting RNA regulons support neuronal growth and regeneration.
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Base de dados:
MEDLINE
Assunto principal:
Axônios
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RNA Mensageiro
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Regulon
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Ribonucleoproteínas Nucleares Heterogêneas
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Motivos de Nucleotídeos
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article