Performance evaluation of an automated image-based fluorescence CD4+ cell analyzer.
Technol Health Care
; 26(5): 867-871, 2018.
Article
em En
| MEDLINE
| ID: mdl-30040773
ABSTRACT
BACKGROUND:
Although AIDS-related mortality has declined since the introduction of antiretroviral therapy (ART), HIV/ AIDS patients are predominantly present in developing countries that lack high-cost diagnostic devices and human expertise.OBJECTIVE:
New methods for counting CD4+ cells cost-effectively are needed to replace conventional flow cytometry-based diagnosis.METHODS:
We developed a CD4+ cell analyzer, ADAMII, which is a benchtop fluorescence image-based CD3+/4+ cell counting analyzer. It bears a three-channel light source and performs CD3+/4+ counting assays. The automatic 3D stage captures a maximum of 136 images that are subsequently processed and analyzed using a software integrated into the system.RESULTS:
Results obtained using ADAMII were compared with data obtained by conventional methods using a FACSCalibur flow cytometer and the point-of-care PIMA CD4 analyzer. Both comparisons between ADAMII vs. FACS and ADAMII vs. PIMA data yielded a strong correlation with an R2 value of 0.98, which ensures the feasibility of CD4 test by ADAMII.CONCLUSIONS:
The proposed method using ADAMII can be easily employed in resource-limited areas to replace conventional flow cytometers, which are expensive and require highly trained staff.Palavras-chave
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Base de dados:
MEDLINE
Assunto principal:
Linfócitos T CD4-Positivos
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Infecções por HIV
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Contagem de Linfócito CD4
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Sistemas Automatizados de Assistência Junto ao Leito
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Citometria de Fluxo
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Fluorescência
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article