Chromatin Immunoprecipitation.
Methods Mol Biol
; 1839: 65-75, 2018.
Article
em En
| MEDLINE
| ID: mdl-30047055
ABSTRACT
Chromatin immunoprecipitation (ChIP) measures the physical association between a protein and DNA in the cell. In combination with next-generation sequencing, the technique enables the identification of DNA targets for the corresponding protein across an entire genome. Here we describe the immunoprecipitation of Vibrio cholerae DNA bound to the histone-like nucleoid structuring protein (H-NS) tagged with the Flag epitope. The quality of the DNA obtained in this protocol is suitable for next-generation sequencing. The procedure described herein can be readily adapted to other bacteria and DNA-binding proteins.
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Base de dados:
MEDLINE
Assunto principal:
Imunoprecipitação da Cromatina
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article