Socioenvironmental stressors encountered during spaceflight partially affect the murine TCR-ß repertoire and increase its self-reactivity.
FASEB J
; 33(1): 896-908, 2019 01.
Article
em En
| MEDLINE
| ID: mdl-30052484
ABSTRACT
Spaceflights are known to affect the immune system. In a previous study, we demonstrated that hypergravity exposure during murine development modified 85% of the T-cell receptor (TCR)-ß repertoire. In this study, we investigated whether socioenvironmental stressors encountered during space missions affect T lymphopoiesis and the TCR-ß repertoire. To address this question, pregnant mice were subjected throughout gestation to chronic unpredictable mild stressors (CUMS), a model used to mimic socioenvironmental stresses encountered during space missions. Then, newborn T lymphopoiesis and the TCR-ß repertoire were studied by flow cytometry and high-throughput sequencing, respectively. No change in thymocyte maturation or TCR expression were noted. TCR-ß repertoire analysis revealed that 75% of neonate TCR-ß sequences resulted from the expression of 3 variable (V)ß segments and that this core repertoire was not affected by CUMS. However, the minor repertoire, representing 25% of the global repertoire, was sensitive to CUMS exposure. We also showed that the variable (diversity) joining [V(D)J] recombination process was unlikely to be affected. Finally, we noted that the CUMS neonatal minor repertoire was more self-reactive than the one of control pups. These findings show that socioenvironmental stressors such as those encountered during space missions affect a fraction (25%) of the TCR-ß repertoire and that these stressors could increase self-reactivity.-Fonte, C., Kaminski, S., Vanet, A., Lanfumey, L., Cohen-Salmon, C., Ghislin, S., Frippiat, J.-P. Socioenvironmental stressors encountered during spaceflight partially affect the murine TCR-ß repertoire and increase its self-reactivity.
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MEDLINE
Assunto principal:
Voo Espacial
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Estresse Fisiológico
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Estresse Psicológico
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Receptores de Antígenos de Linfócitos T alfa-beta
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article