Effects of oridonin on immune cells, Th1/Th2 balance and the expression of BLys in the spleens of broiler chickens challenged with Salmonella pullorum.

ABSTRACT

The aim of this study was to investigate the effects of oridonin (ORI) on the immune cells, Th1/Th2 balance and the expression of B lymphocyte stimulator (BLys) in the spleens of broilers infected with Salmonella pullorum. In a completely randomized design, 300 one-day-old AA male broilers were divided to 5 treatments. The groups included a noninfection control (CON) group receiveed a basal diet; a S. pullorum infect control group received the basal diet; and S. pullorum infect group receiveed the basal diet plus 50, 80, and 100 mg/kg ORI, respectively. The results showed that Salmonella challenge increased the relative weights of the spleen, white blood cell counts, lymphocyte and heterophil percentage, H/L ratio, the concentration and mRNA levels of spleen proinflammatory mediators, including tumor necrosis factor-α (TNF-α), interleukin-2 (IL-2), IL-4, IL-6, and IL-10, as well as the anti-inflammatory target Blys (P < .05), and modulated the Th1/Th2 balance (P < .05). ORI pretreatment decreased the relative weight of the spleen and inhibited the release and expression of these proinflammatory mediators and the anti-inflammatory target BLys. The results suggested that ORI supplementation may have immunosuppressive and multiple modulation effects on activated microglia through modulation of the Th1/Th2 balance and BLys expression.