The protective effects of sesamol in a neonatal rat model of necrotizing enterocolitis.
J Matern Fetal Neonatal Med
; 33(6): 889-894, 2020 Mar.
Article
em En
| MEDLINE
| ID: mdl-30058400
ABSTRACT
Background and aim:
Necrotizing enterocolitis (NEC) is a severe gastrointestinal inflammatory disease associated with high rates of morbidity and mortality. Its pathophysiology includes hypoxic-ischemic injury that may be related to oxygen-derived free radical formation. Sesamol is considered to be an antioxidant and free radical scavenger with anti-inflammatory effects. The aim of this study is to investigate the effects of sesamol in a neonatal rat model of NEC.Materials andmethods:
The study included 1-day-old Wistar albino rat pups (n = 34) that were randomly divided into four groups Group 1 (NEC), group 2 (NEC + intraperitoneal sesamol), group 3 (NEC + oral sesamol), and a control group. NEC was induced by exposure to hypoxia/reoxygenation, following cold stress and hyperosmolar enteral formula feeding. Sesamol 100 mg kg-1 dose-1 was administered intraperitoneally to group 2 and orally to group 3 for 3 days. On day 4 all rats were sacrificed. Histological injuries, the Bcl-2, caspase-3, malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione-peroxidase (GSH-Px) activities were measured in intestinal samples.Results:
The grade of intestinal damage, and Bcl-2 and caspase-3 levels in group 1 were significantly higher than in groups 2 and 3 and the control group, and intestinal damage was significantly more severe in group 1 than in groups 2 and 3. The MDA activity was significantly lower in groups 2 and 3 than in group 1 (112, 89, and 144 nmol mL-1, respectively). Groups 2 and 3 had significantly higher SOD and GSH-Px activities than group 1 (SOD 1.75, 1.74, and 0.89 U mg-1; GSH-Px 114, 121, and 110 nmol of NADPH min-1 mg-1, respectively).Conclusions:
The present findings highlight that sesamol has beneficial effects on intestinal injury in a rat model of NEC through its antioxidant and anti-inflammatory properties.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Fenóis
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Substâncias Protetoras
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Enterocolite Necrosante
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Benzodioxóis
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Anti-Inflamatórios
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Antioxidantes
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article