Creation of unnatural base pairs for genetic alphabet expansion toward synthetic xenobiology.
Curr Opin Chem Biol
; 46: 108-114, 2018 10.
Article
em En
| MEDLINE
| ID: mdl-30059833
ABSTRACT
Artificial extra base pairs (unnatural base pairs, UBPs) expand the genetic alphabet of DNA, thus broadening entire biological systems in the central dogma. UBPs function as third base pairs in replication, transcription, and/or translation, and have created a new research area, synthetic xenobiology, providing genetic engineering tools to generate novel DNAs, RNAs, and proteins with increased functionalities. Several UBPs have been developed and applied to PCR technology, DNA aptamer generation, and semi-synthetic organism creation. Among them, we developed a series of UBPs and demonstrated unique quantitative PCR and high-affinity DNA aptamer generation methods.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
DNA
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Engenharia Genética
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Pareamento de Bases
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Código Genético
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article