Real-time tracking of single-molecule collagenase on native collagen and partially structured collagen-mimic substrates.
Chem Commun (Camb)
; 54(73): 10248-10251, 2018 Sep 11.
Article
em En
| MEDLINE
| ID: mdl-30091759
ABSTRACT
The dynamic interactions of an individual matrix metalloproteinase-1 were imaged and monitored in the presence of either triple-helical or non-triple-helical, partially structured collagen-mimic substrates. The enzyme exhibited ten-fold increased catalytic turnover rates with the structurally modified substrate by skipping the triple-helix unwinding step during the catalytic pathway.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Colágeno
/
Mimetismo Molecular
/
Metaloproteinase 1 da Matriz
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article