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Olive Mild Mosaic Virus Coat Protein and P6 Are Suppressors of RNA Silencing, and Their Silencing Confers Resistance against OMMV.
Varanda, Carla Mr; Materatski, Patrick; Campos, Maria Doroteia; Clara, Maria Ivone E; Nolasco, Gustavo; Félix, Maria do Rosário.
Afiliação
  • Varanda CM; ICAAM-Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Instituto de Investigação e Formação Avançada, Universidade de Évora, Pólo da Mitra, Ap.94, 7006-554 Évora, Portugal. carlavaranda@uevora.pt.
  • Materatski P; ICAAM-Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Instituto de Investigação e Formação Avançada, Universidade de Évora, Pólo da Mitra, Ap.94, 7006-554 Évora, Portugal. pmateratski@uevora.pt.
  • Campos MD; ICAAM-Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Instituto de Investigação e Formação Avançada, Universidade de Évora, Pólo da Mitra, Ap.94, 7006-554 Évora, Portugal. mdcc@uevora.pt.
  • Clara MIE; Departamento de Fitotecnia, ICAAM-Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Escola de Ciências e Tecnologia, Universidade de Évora, Pólo da Mitra, Ap.94, 7006-554 Évora, Portugal. iclara@uevora.pt.
  • Nolasco G; MeditBio-Centro para os Recursos Biológicos e Alimentos Mediterrânicos, Faculdade de Ciências e Tecnologia, Universidade do Algarve, Campus de Gambelas, 8005-139 Faro, Portugal. gnolasco@ualg.pt.
  • Félix MDR; Departamento de Fitotecnia, ICAAM-Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Escola de Ciências e Tecnologia, Universidade de Évora, Pólo da Mitra, Ap.94, 7006-554 Évora, Portugal. mrff@uevora.pt.
Viruses ; 10(8)2018 08 09.
Article em En | MEDLINE | ID: mdl-30096871
ABSTRACT
RNA silencing is an important defense mechanism in plants, yet several plant viruses encode proteins that suppress this mechanism. In this study, the genome of the Olive mild mosaic virus (OMMV) was screened for silencing suppressors. The full OMMV cDNA and 5 OMMV open reading frames (ORFs) were cloned into the Gateway binary vector pK7WG2, transformed into Agrobacterium tumefaciens, and agroinfiltrated into N. benthamiana 16C plants. CP and p6 showed suppressor activity, with CP showing significantly higher activity than p6, yet activity that was lower than the full OMMV, suggesting a complementary action of CP and p6. These viral suppressors were then used to induce OMMV resistance in plants based on RNA silencing. Two hairpin constructs targeting each suppressor were agroinfiltrated in N. benthamiana plants, which were then inoculated with OMMV RNA. When silencing of both suppressors was achieved, a significant reduction in viral accumulation and symptom attenuation was observed as compared to those of the controls, as well as to when each construct was used alone, proving them to be effective against OMMV infection. This is the first time that a silencing suppressor was found in a necrovirus, and that two independent proteins act as silencing suppressors in a virus member of the Tombusviridae family.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Doenças das Plantas / Proteínas Virais / Tombusviridae / Interferência de RNA / Resistência à Doença Idioma: En Ano de publicação: 2018 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Doenças das Plantas / Proteínas Virais / Tombusviridae / Interferência de RNA / Resistência à Doença Idioma: En Ano de publicação: 2018 Tipo de documento: Article