Effects of novel brominated flame retardant TBBPA on human airway epithelial cell (A549) in vitro and proteome profiling.
Environ Toxicol
; 33(12): 1245-1253, 2018 Dec.
Article
em En
| MEDLINE
| ID: mdl-30098271
ABSTRACT
The cellular toxicity response of human airway epithelial cells (A549) to tetrabromobisphenol (TBBPA) was assessed in vitro. Cell viability, levels of intracellular reactive oxygen species (ROS), lipid peroxidation (MDA), and caspase-3 activity were determined after A549 treated with varying concentrations of TBBPA. A comparative proteomic analysis was performed in cells treated with different concentrations of TBBPA (0, 10, and 40 µg/mL). Two-way anova analysis showed that cell viability was significantly decreased after treatment by TBBPA with a concentration of 16 µg/mL for 48 hr, however, the caspase-3 activities, ROS generation, and MDA content increased. Ultrastructural observation revealed that the cell was morphological damaged after exposure to 64 µg/mL TBBPA, with mitochondria seriously injured and the smooth endoplasmic reticulum dilated. There was a good correlation between ROS generation and mitochondrial dysfunction. Seventeen differentially expressed proteins involved in various biological processes were identified. These findings provide a basis for understanding the mechanisms of cell dysfunction and perturbation of antioxidant status induced by additive flame retardant on airway epithelial cells.
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MEDLINE
Assunto principal:
Bifenil Polibromatos
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Mucosa Respiratória
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Proteoma
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Células Epiteliais
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Retardadores de Chama
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article