Generation of patient-specific induced pluripotent stem cell lines from one patient with Jervell and Lange-Nielsen syndrome, one with type 1 long QT syndrome and two healthy relatives.
Stem Cell Res
; 31: 174-180, 2018 08.
Article
em En
| MEDLINE
| ID: mdl-30099333
ABSTRACT
Four human iPSC cell lines (one Jervell and Lange-Nielsen Syndrome, one Long QT Syndrome-type 1 and two healthy controls) were generated from peripheral blood obtained from donors belonging to the same family. CytoTune™-iPS 2.0 Sendai Reprogramming Kit (containing OCT3/4, KLF4, SOX2 and cMYC as reprogramming factors) was used to generate all cell lines. The four iPSCs have normal karyotype, express pluripotency markers as determined by RT-PCR and flow cytometry and differentiated spontaneously in vitro into cells of the three germ layers, confirming their pluripotent capacity.
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Base de dados:
MEDLINE
Assunto principal:
Síndrome do QT Longo
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Síndrome de Jervell-Lange Nielsen
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Células-Tronco Pluripotentes Induzidas
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article