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The wheat TdRL1 is the functional homolog of the rice RSS1 and promotes plant salt stress tolerance.
Mahjoubi, Habib; Tamari, Yutaka; Takeda, Shin; Bouchabké-Coussa, Oumaya; Hanin, Moez; Herzog, Etienne; Schmit, Anne-Catherine; Chabouté, Marie-Edith; Ebel, Chantal.
Afiliação
  • Mahjoubi H; Laboratoire de Biotechnologie et d'Amélioration des Plantes, Centre de Biotechnologie de Sfax, BP 1177, 3018, Sfax, Tunisia.
  • Tamari Y; Institut de biologie moléculaire des plantes, UPR 2357 du CNRS, Université de Strasbourg, 12, rue du Général Zimmer, 67084, Strasbourg Cedex, France.
  • Takeda S; Bioscience and Biotechnology Center, Nagoya University, Chikusa, Nagoya, 464-8601, Japan.
  • Bouchabké-Coussa O; Bioscience and Biotechnology Center, Nagoya University, Chikusa, Nagoya, 464-8601, Japan.
  • Hanin M; Institut Jean-Pierre Bourgin, INRA, AgroParisTech, CNRS, Université Paris-Saclay, 78000, Versailles, France.
  • Herzog E; Plant Physiology and Functional Genomics Research Unit, Institute of Biotechnology, University of Sfax, BP 1175, 3038, Sfax, Tunisia.
  • Schmit AC; Institut de biologie moléculaire des plantes, UPR 2357 du CNRS, Université de Strasbourg, 12, rue du Général Zimmer, 67084, Strasbourg Cedex, France.
  • Chabouté ME; Institut de biologie moléculaire des plantes, UPR 2357 du CNRS, Université de Strasbourg, 12, rue du Général Zimmer, 67084, Strasbourg Cedex, France.
  • Ebel C; Institut de biologie moléculaire des plantes, UPR 2357 du CNRS, Université de Strasbourg, 12, rue du Général Zimmer, 67084, Strasbourg Cedex, France.
Plant Cell Rep ; 37(12): 1625-1637, 2018 Dec.
Article em En | MEDLINE | ID: mdl-30099611
ABSTRACT
KEY MESSAGE Rice rss1 complementation assays show that wheat TdRL1 and RSS1 are true functional homologs. TdRL1 over-expression in Arabidopsis conferred salt stress tolerance and alleviated ROS accumulation. Plants have developed highly flexible adaptive responses to their ever-changing environment, which are often mediated by intrinsically disordered proteins (IDP). RICE SALT SENSITIVE 1 and Triticum durum RSS1-Like 1 protein (TdRL1) are both IDPs involved in abiotic stress responses, and possess conserved D and DEN-Boxes known to be required for post-translational degradation by the APC/Ccdc20 cyclosome. To further understand their function, we performed a computational analysis to compare RSS1 and TdRL1 co-expression networks revealing common gene ontologies, among which those related to cell cycle progression and regulation of microtubule (MT) networks were over-represented. When over-expressed in Arabidopsis, TdRL1GFP was present in dividing cells and more visible in cortical and endodermal cells of the Root Apical Meristem (RAM). Incubation with the proteasome inhibitor MG132 stabilized TdRL1GFP expression in RAM cells showing a post-translational regulation. Moreover, immuno-cytochemical analyses of transgenic roots showed that TdRL1 was present in the cytoplasm and within the microtubular spindle of mitotic cells, while, in interphasic cells, it was rather restricted to the cytoplasm with a spotty pattern at the nuclear periphery. Interestingly in cells subjected to stress, TdRL1 was partly relocated into the nucleus. Moreover, TdRL1 transgenic lines showed increased germination rates under salt stress conditions as compared to wild type. This enhanced salt stress tolerance was associated to an alleviation of oxidative damage. Finally, when expressed in the rice rss1 mutant, TdRL1 suppressed its dwarf phenotype upon salt stress, confirming that both proteins are true functional homologs required for salt stress tolerance in cereals.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas de Plantas / Oryza / Estresse Fisiológico / Triticum / Homologia de Sequência de Aminoácidos / Tolerância ao Sal Idioma: En Ano de publicação: 2018 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas de Plantas / Oryza / Estresse Fisiológico / Triticum / Homologia de Sequência de Aminoácidos / Tolerância ao Sal Idioma: En Ano de publicação: 2018 Tipo de documento: Article